What The Anti-CD3 Does And Offers

by | Dec 7, 2016 | Science & Technology

The Rabbit Anti-Human CD3 Antibody (monoclonal) is for research purposes only and isn’t to be used in diagnostic procedures. It is an antibody that is designed to react with the intracytoplasmic portion of the antigen expressed by the T cells. It works by staining the human T cells in the medulla and cortex of the thymus and also in the lymphoid tissues. It can be used for neoplastic and normal T cells in both paraffin-embedded and formalin-fixed tissues.

Applications and Procedures For the Monoclonal Version

The Monoclonal Rabbit Anti-Human CD3 Antibody is designed for Flow Cytometry, Western Blotting, and IHC (Immunohistochemistry). The positive control for the IHC procedure is the tonsil while the positive control for Western Blotting is the Jurkat Cell Lysate. Likewise, the positive control for Flow Cytometry is the Jurkat Cell Line.

For all applications and procedures, the antibody must be diluted appropriately, though dilution ratios are a recommendation and estimate.

The Rabbit Polyclonal Anti-CD3

The Polyclonal antibody has been tested on humans and is designed to work similarly to the monoclonal version. However, it is only intended for use in Western Blotting and IHC. There are four purchase methods, including 0.1 ml, 0.5 ml, 1.0 ml, and 7.0 ml options.

Applications/Procedures For The Polyclonal Version

The applications and methods for this version are similar to that of the monoclonal version, though it isn’t used for Flow Cytometry. Likewise, dilution methods may be slightly different, as well as incubation periods.

Storage And Warnings

Both Anti-CD3 varieties should be stored between two and eight degrees Celsius and should never be frozen. It must be stored properly to ensure that the reagent remains stable until the date indicated. Because it is hard to determine instability, positive and negative controls must be tested simultaneously when using unknown specimens.

The Anti-CD3 is an excellent option for testing. Visit Spring Bioscience now to learn more.

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