How To Use Synaptophysin

Synaptophysin is used to label normal neuroendocrine cells of the carotid body, skin, thyroid, pituitary gland, adrenal medulla, pancreas, lung, Paneth’s cells of the gastrointestinal tract, gastric parietal cells, and gastrointestinal mucosa. It can also be used to label the neurons in the spinal cord, retina, and brain. The anti-synaptophysin antibody can also react with the epithelial and neural neuroendocrine neoplasms, such as non-chromaffin paragangliomas, ganglioneuromas, pheo-chromocytomas, and neuroblastomas, among others.

More About It

This antibody is designed only for research and not diagnostic testing. It has no known clone, and the immunogen is the synthetic peptide of synaptophysin in humans. The isotype is the Rabbit IgG, and it has an undetermined epitope. Its molecular weight is 38kDa, and it has been tested on humans. It is only designed to be used for Immunohistochemistry applications.

IHC Application Procedure

To prepare your specimen, you should use paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are essential and should be cleaned with xylene, an alternative to xylene, or graded alcohols. If you are used the concentrated version of the product, you’ll want to dilute the antibody using a ratio of one to 300. However, you can find pre-diluted options available, depending on your needs. Plus, dilutions are estimates, and you should follow your methods and protocols.

To retrieve the antigen, you’ll need to boil your tissue sections in a 10mM citrate buffer with a pH of 6.0 for at least ten minutes. After it has boiled, you’ll want to cool it to room temperature for 20 minutes before proceeding. Once it has boiled and cooled, you will need to incubate for at least 10 minutes at room temperature.

You should always wash the slides between steps and detect the antibody as per the instructions. The positive control is the pheochromocytoma or pancreas with cellular localization occurring in the cytoplasm.

Synaptophysin can be an excellent label for a variety of areas in the body. Visit Spring BioScience to learn more.

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